Parameter: fragment_bin_size


Determines the accuracy of the scoring algorithm with smaller bins being more strict in determining matches between theoretical and observed spectral peaks. Low-resolution spectra require larger bin sizes to accommodate errors in mass accuracy of the observed peaks. Smaller bins also require more system memory, so caution must be exercised when setting this value for high-resolution spectra. For ion trap (low-res) MS/MS spectra, the recommended values is 1.0005. For high-res MS/MS, the recommended value is 0.03.




To indicate the bin size for high-resolution MS/MS spectra:

fragment_bin_size = 0.03

To indicate the bin size for low-resolution MS/MS spectra:

fragment_bin_size = 1.0005