Parameter: mono_link

Overview

Specifies the sites of incomplete cross-linking (i.e. a mono-link) and mass modification. Two values follow this parameter, separated by spaces. The first value is one or more amino acid letters (uppercase only) that can be linked. Use lowercase ‘n’ or ‘c’ if the linker can bind the protein termini. The second value is the net mass of the incomplete cross-link reaction. The mass can be any real number, positive or negative. If multiple mono-links are possible (e.g. with a heterobifunctional cross-linker), use multiple mono_link lines in the configuration file, one for each possible product.

Syntax

[Amino Acid(s)] [Mass]

Version

1.5.0 - present

Example

mono_link  =  nK  156.0786

Deprecated Syntax for versions 1.0 - 1.4.3

[Chem Code] [Mass]

Chem Codes

• 1 = amine (K or n-terminus)
• 2 = carboxyl (D, E, or c-terminus)
• 3 = sulfhydryl (C)
• 4 = acyl (Q)